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Plasmid

Part:BBa_K2765019:Design

Designed by: Zhuanzhuan Pu   Group: iGEM18_BIT-China   (2018-10-04)


Yno1-sgRNA--pSB1C3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2029
    Illegal SpeI site found at 2436
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2029
    Illegal NheI site found at 2184
    Illegal SpeI site found at 2436
    Illegal NotI site found at 2035
    Illegal NotI site found at 2443
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2029
    Illegal XhoI site found at 1013
    Illegal XhoI site found at 1905
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2029
    Illegal SpeI site found at 2436
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2029
    Illegal XbaI site found at 2044
    Illegal SpeI site found at 2436
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is an xba1 restriction site that requires mutations


Source

All from the yeast genome

References